
Abstract
Does it summarize the lab report so that the reader can understand the purpose and results of the project as well as what experimental methods were used to obtain said results and the overall conclusions drawn by the author.
Introduction
The purpose of the project. Provide background information (including previous research by other scientists). Briefly describe experimental outline as well as results.
Materials and methods
List out all materials used for the experiment and an accurate description of all experimental procedures (include all methodologies).
Results
Describe results from your experiments. Include all pictures (gels, photos etc.) with accurate labels and all data tables.
Conclusions
Analyze your results and interpret your collected data. What do your results tell you? What future experiments would you consider doing to further validate your results? What possible improvements could be done with your methods of collecting data?
Citations
Properly cite all source material. APA format with in text citation.
This what I did in lab.
DNA Forensics
Introduction: DNA fingerprinting is a widely used approach to identify organisms and
determine evolutionary relatedness. As an example, DNA fingerprinting is commonly
used in forensics to identify criminals from a set of possible suspects. In short, residual
traces of DNA are collected from a crime scene along with DNA samples from multiple
suspects. DNA fingerprints are then constructed from the crime scene DNA and
compared to all other DNA samples.
Procedure: For each gel, combine 50 ml of TBE buffer with 0.5 g agarose. Swirl mixture.
1. Microwave mixture for 30 sec – 1 min. Using protective gloves, remove the
mixture from the microwave and swirl.. The agarose should be completely
dissolved in the buffer solution and look clear. Make sure that the solution does
not spill out of the flask when boiling.
2. Add 5 ul of SYBR safe solution to each flask and swirl. This is the stain that will
help you visualize the DNA in your gel.
3. Gently pour solution into a gel casting tray and place one comb into the gel.
4. Wait for the gel to solidify
5. Remove your restriction digests from the refrigerator.
6. Add 5 ul of loading dye to each sample.
7. carefully transfer the gel to an electrophoresis chamber. Make sure to fill the
chamber with enough buffer to cover your gel. Also, make sure that the wells of
the gel are near the black electrode (not the red).
8. Load the gels